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pAAV-Ku70-TC
(Plasmid #46955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46955 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-MCS2
  • Backbone size w/o insert (bp) 4650
  • Total vector size (bp) 7319
  • Vector type
    AAV ; r

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XRCC6 targeting construct
  • Alt name
    Ku70
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4426
  • GenBank ID
    NT_011520
  • Entrez Gene
    XRCC6 (a.k.a. CTC75, CTCBF, G22P1, KU70, ML8, TLAA)
  • Tag / Fusion Protein
    • GFP-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI/Bsp12OI (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ku70-TC was a gift from Steve Jackson (Addgene plasmid # 46955 ; http://n2t.net/addgene:46955 ; RRID:Addgene_46955)
  • For your References section:

    A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. Britton S, Coates J, Jackson SP. J Cell Biol. 2013 Jul 29. 10.1083/jcb.201303073 PubMed 23897892