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pBMN YFP-ltDHFR(DD)
(Plasmid #47080)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47080 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Garry Nolan
  • Backbone size w/o insert (bp) 6943
  • Vector type
    Retroviral
  • Selectable markers
    HcRed Fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YFP-ltDHFR(DD)
  • Alt name
    folA dihydrofolate reductase ( destable domain) with YFP fusion for low temperature
  • Species
    E. coli
  • Insert Size (bp)
    1233
  • Mutation
    amino acids mutations: R12H, N37D, N59D, G67S, D132G
  • GenBank ID
    AAC73159
  • Promoter LTR
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XnoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer lab32
  • 3′ sequencing primer LC47
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This C-terminal ltDHFR(DD) plasmid is the clone#7 in Table 1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN YFP-ltDHFR(DD) was a gift from Thomas Wandless (Addgene plasmid # 47080)
  • For your References section:

    Rapid and Tunable Control of Protein Stability in Caenorhabditis elegans Using a Small Molecule. Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ. PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393. 10.1371/journal.pone.0072393 PubMed 23991108