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pcDNA-dCas9
(Plasmid #47106)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47106 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 9575
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    S. Pyogenes
  • Insert Size (bp)
    4236
  • Mutation
    D10A, H840A (catalytically inactive)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-dCas9 was a gift from Charles Gersbach (Addgene plasmid # 47106)
  • For your References section:

    RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. 10.1038/nmeth.2600 PubMed 23892895