pSPgRNA
(Plasmid #47108)

• Purpose: Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells
• Depositing Lab: Charles Gersbach
• Publication: Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.

Backbone

• Vector backbone: pZDonor
• Backbone manufacturer: Sigma
• Total vector size (bp): 3223
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SPgRNA
• Alt name: S. Pyogenes guide RNA
• Species: Synthetic
• Promoter: U6

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: hU6-F
• 3′ sequencing primer: T3

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 92 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/

Information for Cloning Grade DNA (Catalog # 47108-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here