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pcDNA5-EGFP-AID-BubR1
(Plasmid #47330)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47330 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 9850
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Alt name
    Bub1B
  • Alt name
    BubR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4590
  • Mutation
    siRNA resistant
  • GenBank ID
    NM_001211.5
  • Entrez Gene
    BUB1B (a.k.a. BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1)
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • AID (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer ACT AGT GGA TCC CTA TCA CTG AAA GAG CAA AGC CCC AGG ACT AGT TAA CT
  • 3′ sequencing primer ACT CGA GCG GCC GCC TAT CAC TGA AAG AGC AAA GCC CCA GGA CTA GTT AA
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The AID ORF was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
  • Articles Citing this Plasmid

Terms and Licenses

Depositor Comments

Nishimura et al., 2009, Nature Methods

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-EGFP-AID-BubR1 was a gift from Don Cleveland (Addgene plasmid # 47330 ; http://n2t.net/addgene:47330 ; RRID:Addgene_47330)
  • For your References section:

    Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Holland AJ, Fachinetti D, Han JS, Cleveland DW. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12. 10.1073/pnas.1216880109 PubMed 23150568