Addgene - VL3 (Flag-Mad2) Plasmid Data

Plasmid 16047: VL3 (Flag-Mad2)

Gene/insert name:
Mad2
Insert size:
600
Species:
H. sapiens (human)
Entrez Gene:
MAD2L1 (HSMAD2, MAD2)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pFlag-CMV2
(Search Vector Database)
Backbone manufacturer:
Sigma
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Robert Benezra
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mad2 phosphorylation regulates its association with Mad1 and the APC/C. Wassmann et al (EMBO J. 2003 Feb 17. 22(4):797-806. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16047" in your Materials and Methods section.