Addgene - cdk1 FRET sensor (2327) Plasmid Data

Plasmid 26064: cdk1 FRET sensor (2327)

Gene/insert name:
Cdk1 FRET Sensor
Fusion protein or tag:
YFP variant
Terminal:
N terminal on insert
Fusion protein or tag:
CFP variant
Terminal:
C terminal on insert
Fusion protein or tag:
FLAG
Terminal:
C terminal on insert
Vector backbone:
pEYFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Jonathon Pines
Terms and Licenses:
MTA

Comments:

Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.

Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Gavet et al (Dev Cell. 2010 Apr 20. 18(4):533-43. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26064" in your Materials and Methods section.