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p-cyclinB1-mcherry (1958)
(Plasmid #26063)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26063 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    See note below
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • mcherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mCherry-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Depositor Comments

The backbone was derived from pECFP-N1. ECFP was replaced with mCherry.

There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-cyclinB1-mcherry (1958) was a gift from Jonathon Pines (Addgene plasmid # 26063 ; http://n2t.net/addgene:26063 ; RRID:Addgene_26063)
  • For your References section:

    Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Gavet O, Pines J. Dev Cell. 2010 Apr 20. 18(4):533-43. 10.1016/j.devcel.2010.02.013 PubMed 20412769