|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26063||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSee note below
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCyclin B1
SpeciesH. sapiens (human)
Entrez GeneCCNB1 (a.k.a. CCNB)
/ Fusion Protein
- mcherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The backbone was derived from pECFP-N1. ECFP was replaced with mCherry.
There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p-cyclinB1-mcherry (1958) was a gift from Jonathon Pines (Addgene plasmid # 26063 ; http://n2t.net/addgene:26063 ; RRID:Addgene_26063)
For your References section:Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Gavet O, Pines J. Dev Cell. 2010 Apr 20. 18(4):533-43. 10.1016/j.devcel.2010.02.013 PubMed 20412769