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cyclin B1-CDK1 FRET biosensor
(Plasmid #52097)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52097 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Modifications to backbone
    Kozac sequence has been added before insert gene and XhoI restriction site. The reading frame of XhoI site has also been modified.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDK1 biosensor
  • Alt name
    TFP-FHA2-Substrate-YFP
  • Species
    Synthetic; synthetic construct
  • Insert Size (bp)
    1986
  • GenBank ID
    KF985963
  • Promoter CMV
  • Tag / Fusion Protein
    • Kozac sequence (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Addgene's quality control sequence shows that there is a Q to M mutation in the sensor. The mutations should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cyclin B1-CDK1 FRET biosensor was a gift from Robert Campbell (Addgene plasmid # 52097)
  • For your References section:

    Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1. Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE. Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e. 10.1039/c3mb70402e PubMed 24281384