cyclin B1-CDK1 FRET biosensor
PurposeA FRET-based biosensors for cyclin dependent kinase 1 (CDK1) in complex with cyclin B1, with improved ratio change. It is for monitoring cyclin B1-CDK1 kinase activity in live cells.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52097||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7500
Modifications to backboneKozac sequence has been added before insert gene and XhoI restriction site. The reading frame of XhoI site has also been modified.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCDK1 biosensor
SpeciesSynthetic; synthetic construct
Insert Size (bp)1986
- Promoter CMV
/ Fusion Protein
- Kozac sequence (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Terms and Licenses
Please note that Addgene's quality control sequence shows that there is a Q to M mutation in the sensor. The mutations should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:cyclin B1-CDK1 FRET biosensor was a gift from Robert Campbell (Addgene plasmid # 52097)
For your References section:Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1. Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE. Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e. 10.1039/c3mb70402e PubMed 24281384