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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) Citations (4)

Plasmid Article: Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA Proc Natl Acad Sci U S A. 2013 Aug 12.
PubMed Journal

Articles Citing pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Articles
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells. Lee CM, Cradick TJ, Bao G. Mol Ther. 2016 Mar;24(3):645-54. doi: 10.1038/mt.2016.8. Epub 2016 Jan 19. PubMed
Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Yang D, Scavuzzo MA, Chmielowiec J, Sharp R, Bajic A, Borowiak M. Sci Rep. 2016 Feb 18;6:21264. doi: 10.1038/srep21264. PubMed
NmeCas9 is an intrinsically high-fidelity genome-editing platform. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ. Genome Biol. 2018 Dec 5;19(1):214. doi: 10.1186/s13059-018-1591-1. PubMed

Associated Plasmids

AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage. Song G, Zhang F, Zhang X, Gao X, Zhu X, Fan D, Tian Y. Cell Rep. 2019 Nov 26;29(9):2579-2589.e4. doi: 10.1016/j.celrep.2019.10.078. PubMed

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