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pT7goldRNA
(Plasmid #47930)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47930 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT7-gRNA
  • Backbone size w/o insert (bp) 2542
  • Total vector size (bp) 2526
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    golden gRNA
  • Alt name
    golden
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    20
  • Entrez Gene
    slc24a5 (a.k.a. CH1073-436O12.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer M13 Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/

gRNA target sequence GGTCTCTCGCAGGATGTTGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7goldRNA was a gift from Wenbiao Chen (Addgene plasmid # 47930 ; http://n2t.net/addgene:47930 ; RRID:Addgene_47930)
  • For your References section:

    Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Jao LE, Wente SR, Chen W. Proc Natl Acad Sci U S A. 2013 Aug 5. 10.1073/pnas.1308335110 PubMed 23918387