pCH044
(Plasmid
#47950)
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PurposertTA under the control of GAL2 promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS306
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Backbone manufacturerATCC
- Total vector size (bp) 4373
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Vector typeYeast Expression ; Tet inducible expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namertTA
- Promoter Gal2
Cloning Information
- Cloning method Unknown
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TetR is downstream of Gal2 promoter.
The discrepancies found between the Addgene QC sequence and the full sequence do not affect the functioning of the construct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCH044 was a gift from Attila Becskei (Addgene plasmid # 47950 ; http://n2t.net/addgene:47950 ; RRID:Addgene_47950) -
For your References section:
Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution. Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, Becskei A. Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687. 10.1038/ncomms1687 PubMed 22353713
