Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #48122)


Item Catalog # Description Quantity Price (USD)
Plasmid 48122 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Malten et al., 2005
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    For Bacillus megaterium: Tetracycline and 37 °C
  • Copy number


  • Gene/Insert name
    predicted optimal signal peptide for Bacillus megaterium protein secretion

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPAsp-hp was a gift from Dieter Jahn (Addgene plasmid # 48122 ; ; RRID:Addgene_48122)
  • For your References section:

    High-yield intra- and extracellular protein production using Bacillus megaterium. Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30. 10.1128/AEM.00431-10 PubMed 20435764