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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
(Plasmid #48253)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48253 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV_hSyn_WPRE_bGHpA
  • Backbone size w/o insert (bp) 4281
  • Total vector size (bp) 6771
  • Vector type
    Mammalian Expression, AAV ; TALE

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2PHR-NLS-VP64
  • Species
    Synthetic
  • Insert Size (bp)
    2490
  • Promoter EF1-alpha
  • Tag / Fusion Protein
    • 2A_GFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA was a gift from Feng Zhang (Addgene plasmid # 48253)
  • For your References section:

    Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F. Nature. 2013 Jul 23. doi: 10.1038/nature12466. 10.1038/nature12466 PubMed 23877069