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pTrex-Luc-Phleo
(Plasmid #48337)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48337 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pTrex
  • Backbone manufacturer
    Martin P. Vazquez
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 7900
  • Modifications to backbone
    Luciferase inserted in MCS through HindIII and XhoI.
  • Vector type
    Trypanasoma cruzi expression
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    firely luciferase
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1700
  • Promoter T. cruzi rRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrex-Luc-Phleo was a gift from Rick Tarleton (Addgene plasmid # 48337 ; http://n2t.net/addgene:48337 ; RRID:Addgene_48337)