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AAV GFP
(Plasmid #49055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49055 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    adeno-associated viral (AAVsp)
  • Backbone size w/o insert (bp) 6204
  • Total vector size (bp) 7074
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    Enhanced Green Fluorescent Protein
  • Species
    Aequorea victoria green
  • Insert Size (bp)
    720
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer tagccacaccagccaccact
  • 3′ sequencing primer caacgtgctggtctgtgtg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Expression of GFP is driven by a CMV promoter with a splice donor/acceptor sequence (SD/SA) and flanked by inverted terminal repeats (ITRs).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV GFP was a gift from Fred Gage (Addgene plasmid # 49055 ; http://n2t.net/addgene:49055 ; RRID:Addgene_49055)
  • For your References section:

    Adeno-associated virus effectively mediates conditional gene modification in the brain. Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2320-5. Epub 2002 Feb 12. 10.1073/pnas.042678699 PubMed 11842206