|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49101||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCallaway Addgene 26197
- Total vector size (bp) 8022
Modifications to backboneReplaced GFP-TVA in pAAV-EF1a-FLEX-GTB with Cerulean
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Alt namerabies virus glycoprotein
Insert Size (bp)2367
- Promoter EF1a
/ Fusion Protein
- cerulean (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer atttgccctttttgagtttgg
- 3′ sequencing primer CAAGGCTGGTGGGCACTGG (Common Sequencing Primers)
GFP-2A-TVA in pAAV-EF1a-Flex-GTB is replaced with cerulean.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-FLEX-C-RG was a gift from Troy Margrie (Addgene plasmid # 49101 ; http://n2t.net/addgene:49101 ; RRID:Addgene_49101)