PurposeAAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49129||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberLow Copy
- Promoter DBH
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
For additional information, please see the following publications:
Hwang DY et al., 2005. Genetically engineered dopamine beta-hydroxylase gene promoters with better phox2-binding sites drive significantly enhanced transgene expression in a noradrenergic cell-specific manner. Mol Ther 11, 132-141, PMID 15585414 (https://www.ncbi.nlm.nih.gov/pubmed/15585414).
de Leeuw CN et al., rAAV-compatible MiniPromoters for restricted expression in the brain and eye. Mol Brain. 2016 May 10;9(1):52, PMID 27164903 (https://www.ncbi.nlm.nih.gov/pubmed/27164903).
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2022 was a gift from Elizabeth Simpson (Addgene plasmid # 49129 ; http://n2t.net/addgene:49129 ; RRID:Addgene_49129)
For your References section:rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 [pii] PubMed 27164903