|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49356||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameAmeR repressor
MutationD120F, R121Q, G123H, Y124T, E125G, M126F, W127YS
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRF-AmeR was a gift from Christopher Voigt (Addgene plasmid # 49356 ; http://n2t.net/addgene:49356 ; RRID:Addgene_49356)
For your References section:Genomic mining of prokaryotic repressors for orthogonal logic gates. Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. 10.1038/nchembio.1411 PubMed 24316737