Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Genomic mining of prokaryotic repressors for orthogonal logic gates.
Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
PubMed Article

Deposited by Christopher Voigt's lab, these response function plasmids (pRF-) contain a transcriptional repressor, which controls the expression of a YFP output. Repressors are under the control of the IPTG-inducible Ptac promoter. Expression of the YFP gene is controlled by a synthetic constitutive promoter that contains a repressor operator sequence. The YFP output is repressed in the presence of IPTG. The various circuit plasmids achieve either the NAND (pLux-LmrA and pNAND-PhlF) or AND (pAND) behavior. To generate the NAND behavior, the following input concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 20 µM 3OC6HSL (-/+), and 1 mM IPTG and 20 µM 3OC6HSL (+/+).To generate the AND behavior, the following inducer concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 100 ng/mL aTc (-/+), and 1 mM and 100 ng/mL aTc (+/+).

Voigt Lab Repressors for Orthogonal Logic Gates

Plasmids from Article

ID Plasmid  
49355pRF-AmtR
Loading...
49356pRF-AmeR
Loading...
49357pRF-BetI
Loading...
49358pRF-BM3R1
Loading...
49359pRF-ButR
Loading...
49360pRF-HapR
Loading...
49361pRF-HlyIIR
Loading...
49362pRF-IcaR
Loading...
49363pRF-LitR
Loading...
49364pRF-LmrA
Loading...
49365pRF-McbR
Loading...
49366pRF-Orf2
Loading...
49367pRF-PhlF
Loading...
49368pRF-PsrA
Loading...
49369pRF-QacR
Loading...
49370pRF-ScbR
Loading...
49371pRF-SmcR
Loading...
49372pRF-SrpR
Loading...
49373pRF-TarA
Loading...
49374pRF-TetR
Loading...
49375pLux-LmrA
Loading...
49376pNAND-PhlF
Loading...
49377pAND
Loading...
51148pET21a-AcrR
Loading...
51149pET21a-AmeR
Loading...
51150pET21a-AmrR
Loading...
51151pET21a-AmtR
Loading...
51154pET21a-BarA
Loading...
51155pET21a-BarB
Loading...
51156pET21a-BetI
Loading...
51157pET21a-BM1P1
Loading...
51158pET21a-BM3R1
Loading...
51159pET21a-BpeR
Loading...
51161pET21a-CalR1
Loading...
51162pET21a-CampR
Loading...
51163pET21a-CasR
Loading...
51164pET21a-CprB
Loading...
51165pET21a-CymR
Loading...
51166pET21a-Cyp106
Loading...
51167pET21a-DhaR
Loading...
51168pET21a-Ef0113
Loading...
51170pET21a-EthR
Loading...
51172pET21a-HapR
Loading...
51173pET21a-HemR
Loading...
51174pET21a-HlyIIR
Loading...
51176pET21a-IcaR-e
Loading...
51177pET21a-IfeR
Loading...
51178pET21a-JadR2
Loading...
51179pET21a-KstR
Loading...
51181pET21a-LitR
Loading...
51183pET21a-LuxT
Loading...
51184pET21a-McbR
Loading...
51188pET21a-MtrR
Loading...
51189pET21a-NonG
Loading...
51191pET21a-Orf2
Loading...
51192pET21a-orfL6
Loading...
51193pET21a-PaaR
Loading...
51195pET21a-PqrA
Loading...
51210pET21a-TylP
Loading...