pcDNA5-F-AF9
(Plasmid #49428)

• Purpose: Mammalian expression of Flag-tagged human AF9
• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	49428	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pcDNA5
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AF-9
• Species: H. sapiens (human)
• GenBank ID: NP_004520.2 IMAGE clone: 5298142
• Entrez Gene: MLLT3 (a.k.a. AF9, YEATS3)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 2 References