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FH-TET3-pEF
(Plasmid #49446)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49446 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1a
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 11669
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet methylcytosine dioxygenase 3
  • Alt name
    TET3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5469
  • Entrez Gene
    TET3 (a.k.a. hCG_40738)
  • Promoter EF-1alpha
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH-TET3-pEF was a gift from Anjana Rao (Addgene plasmid # 49446 ; http://n2t.net/addgene:49446 ; RRID:Addgene_49446)
  • For your References section:

    Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX. Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6. doi: 10.1038/nature12052. Epub 2013 Apr 7. 10.1038/nature12052 PubMed 23563267