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pCMV5-hBMPR-1A CA
(Plasmid #49527)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49527 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4657
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BMPR-1A CA
  • Alt name
    BMPR-1A
  • Alt name
    Alk3
  • Species
    H. sapiens (human)
  • Mutation
    constitutively active mutation (Q233D); also P2A and K208R
  • Entrez Gene
    BMPR1A (a.k.a. 10q23del, ACVRLK3, ALK3, CD292, SKR5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The BMPR-1A insert in this plasmid contains P2A and K208R mutations when compared to GenBank reference sequence NP_004320.2. These mutations are not a concern for the function of the plasmid, as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-hBMPR-1A CA was a gift from Lee Niswander & Peter ten Dijke (Addgene plasmid # 49527 ; http://n2t.net/addgene:49527 ; RRID:Addgene_49527)
  • For your References section:

    Expression of a constitutively active type I BMP receptor using a retroviral vector promotes the development of adrenergic cells in neural crest cultures. Varley JE, McPherson CE, Zou H, Niswander L, Maxwell GD. Dev Biol. 1998 Apr 1;196(1):107-18. 10.1006/dbio.1998.8853 PubMed 9527884