|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49527||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDr. David Russell, UTSW
- Backbone size w/o insert (bp) 4657
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameBMPR-1A CA
SpeciesH. sapiens (human)
Mutationconstitutively active mutation (Q233D); also P2A and K208R
Entrez GeneBMPR1A (a.k.a. 10q23del, ACVRLK3, ALK3, CD292, SKR5)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
The BMPR-1A insert in this plasmid contains P2A and K208R mutations when compared to GenBank reference sequence NP_004320.2. These mutations are not a concern for the function of the plasmid, as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5-hBMPR-1A CA was a gift from Lee Niswander & Peter ten Dijke (Addgene plasmid # 49527 ; http://n2t.net/addgene:49527 ; RRID:Addgene_49527)
For your References section:Expression of a constitutively active type I BMP receptor using a retroviral vector promotes the development of adrenergic cells in neural crest cultures. Varley JE, McPherson CE, Zou H, Niswander L, Maxwell GD. Dev Biol. 1998 Apr 1;196(1):107-18. 10.1006/dbio.1998.8853 PubMed 9527884