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(Plasmid #49530)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 49530 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2959
  • Total vector size (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    BMPR-1B DN
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    dominant negative mutation (K231R); also L2V
  • Entrez Gene
    Bmpr1b (a.k.a. AI385617, ALK-6, AV355320, Acvrlk6, Alk6, BMPR-1B, BMPR-IB, CFK-43a, SKR6)
  • Promoter T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The BMPR-1B insert in this plasmid contains a L2V mutation when compared to GenBank reference sequence NP_031586.1. This mutation is not a concern for the function of the plasmid, as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSK+ mBMPR-1B DN was a gift from Lee Niswander & Peter ten Dijke (Addgene plasmid # 49530 ; ; RRID:Addgene_49530)
  • For your References section:

    Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage. Zou H, Wieser R, Massague J, Niswander L. Genes Dev. 1997 Sep 1;11(17):2191-203. 10.1101/gad.11.17.2191 PubMed 9303535