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EB3-tdTomato
(Plasmid #50708)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50708 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    tdTomato-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB3
  • Alt name
    MAPRE3
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

We received the EB3 construct from Niels Galjart (Erasmus U, Netherlands) originally and replaced GFP with tdTomato.
There is a Sal I site at the 5' end of EB3 and a Not I site at the 3' end of tdTomato.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708 ; http://n2t.net/addgene:50708 ; RRID:Addgene_50708)
  • For your References section:

    Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and drebrin. Merriam EB, Millette M, Lumbard DC, Saengsawang W, Fothergill T, Hu X, Ferhat L, Dent EW. J Neurosci. 2013 Oct 16;33(42):16471-82. doi: 10.1523/JNEUROSCI.0661-13.2013. 10.1523/JNEUROSCI.0661-13.2013 PubMed 24133252