|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50825||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 6216
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt namemicrotubule-associated protein, RP/EB family, member 1
SpeciesM. musculus (mouse)
Entrez GeneMapre1 (a.k.a. 5530600P05Rik, AI462499, AI504412, AW260097, BIM, BIM1p, D2Ertd459, D2Ertd459e, Eb1)
- Promoter CMV
/ Fusion Protein
- tdTomato (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
We received the EB1 construct from Yuko Mimori-Kiyosue (RIKEN, Kobe, Japan) originally and replaced the GFP with tdTomato.
There is a 4x Gly linker between the EB1 and the tdTomato.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EB1-tdTomato was a gift from Erik Dent (Addgene plasmid # 50825 ; http://n2t.net/addgene:50825 ; RRID:Addgene_50825)