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EB1-tdTomato
(Plasmid #50825)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50825 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    tdTomato-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6216
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Alt name
    microtubule-associated protein, RP/EB family, member 1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mapre1 (a.k.a. 5530600P05Rik, AI462499, AI504412, AW260097, BIM1p, D2Ertd459e, Eb1)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

We received the EB1 construct from Yuko Mimori-Kiyosue (RIKEN, Kobe, Japan) originally and replaced the GFP with tdTomato.
There is a 4x Gly linker between the EB1 and the tdTomato.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EB1-tdTomato was a gift from Erik Dent (Addgene plasmid # 50825 ; http://n2t.net/addgene:50825 ; RRID:Addgene_50825)