Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAcBac2.tR4-OMeYRS/GFP*
(Plasmid #50831)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50831 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcBac
  • Modifications to backbone
    derived from pFastBac-Dual from Invitrogen.
  • Vector type
    Insect Expression ; baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    two-copy Tyr tRNA cassette
  • Species
    Synthetic
  • Promoter U6 and H1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    EGFP*
  • Mutation
    Y39TAG
  • Promoter CAG
  • Tags / Fusion Proteins
    • WPRE (C terminal on insert)
    • Myc-6xHis (C terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    OMeYRS
  • Alt name
    E. coli tyrosyl-tRNA synthetase
  • Species
    E. coli
  • Mutation
    able to charge various unnatural amino acids (UAAs) but not canonical amino acids. Y37V, D182S, F183M, D265R
  • Promoter CMV
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    two copy Tyr tRNA cassette
  • Species
    Synthetic
  • Promoter U6 and H1

Cloning Information for Gene/Insert 4

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac2.tR4-OMeYRS/GFP* was a gift from Peter Schultz (Addgene plasmid # 50831 ; http://n2t.net/addgene:50831 ; RRID:Addgene_50831)
  • For your References section:

    Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 10.1073/pnas.1309584110. Epub 2013 Jul 1. 10.1073/pnas.1309584110 PubMed 23818609