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pEVOL-pBpF
(Plasmid #31190)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31190 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Any strain for growth, 37 oC, LB/2XYT media
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
  • Alt name
    pEVOL-pBpF
  • Species
    M.jannaschii
  • Insert Size (bp)
    1000
  • Mutation
    Y32G, E107P, D158T, I159S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-pBpF was a gift from Peter Schultz (Addgene plasmid # 31190 ; http://n2t.net/addgene:31190 ; RRID:Addgene_31190)
  • For your References section:

    Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli. Chin JW, Martin AB, King DS, Wang L, Schultz PG. Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11020-4. Epub 2002 Aug 1. 10.1073/pnas.172226299 PubMed 12154230