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pULTRA-CNF
(Plasmid #48215)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48215 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pULTRA
  • Backbone manufacturer
    Schultz lab
  • Modifications to backbone
    The Clodf13 replicon, spectinomycin resistance cassette and the lacI gene of pUltra were derived from pCDF-1b(Novagen). The T7 promoter/terminator elements in pCDF-1b were replaced with a tacI promoter sequence followed by a multiple-cloning site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    polyspecific MJ tyrosyl synthetase
  • Species
    M. jannaschii
  • Mutation
    Y32L, L65V, F108W, Q109M, D158G, I159A
  • Promoter tacI

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer Tac promoter
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tRNA
  • Promoter proK

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (unknown if destroyed)
  • 3′ cloning site NcoI (unknown if destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid was also used in Chatterjee et al Biochemistry. 2013 Feb 27. PMID: 23379331

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pULTRA-CNF was a gift from Peter Schultz (Addgene plasmid # 48215 ; http://n2t.net/addgene:48215 ; RRID:Addgene_48215)
  • For your References section:

    A genetically encoded infrared probe. Schultz KC, Supekova L, Ryu Y, Xie J, Perera R, Schultz PG. J Am Chem Soc. 2006 Nov 1;128(43):13984-5. 10.1021/ja0636690 PubMed 17061854