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C321.ΔA.exp
(Bacterial strain #49018)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 49018 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin(Zeocin)
  • Growth Temperature
    30°C
  • Growth Strain(s)
    this strain
  • Growth instructions
    This strain has mutS+ (reduced mutation rate compared to C321.deltaA) and lambda- (growth can be performed at 37C). This strain must be supplemented with d-biotin when grown in minimal media.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
  • Alt name
    C321.deltaA.exp
  • Alt name
    C321.ΔA.exp
  • Species
    E. coli
  • Insert Size (bp)
    4633801
  • Mutation
    See genbank file
  • GenBank ID

Resource Information

Depositor Comments

E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA

This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.

The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.ΔA.exp was a gift from George Church (Addgene plasmid # 49018)
  • For your References section:

    Genomically recoded organisms expand biological functions. Lajoie MJ, Rovner AJ, Goodman DB, Aerni HR, Haimovich AD, Kuznetsov G, Mercer JA, Wang HH, Carr PA, Mosberg JA, Rohland N, Schultz PG, Jacobson JM, Rinehart J, Church GM, Isaacs FJ. Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459. 10.1126/science.1241459 PubMed 24136966