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C321
(Bacterial strain #48999)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 48999 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    this strain
  • Growth instructions
    Growth above 34 C is not recommended, since growth at elevated temperatures leads to leaky expression of toxic proteins from the lambda prophage. A lambda- version will be deposited at addgene soon.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Recoded E. coli genome with all instances of the UAG codon removed, but wild type UAG termination function is not changed
  • Species
    E. coli
  • Mutation
    See genbank file of C321.DA
  • GenBank ID

Resource Information

Depositor Comments

E. coli MG1655 delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta mutS::zeoR; all 321 UAG codons changed to UAA

This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.

Sequence is similar to C321.DA (NCBI accession # NCBI CP006698), except that RF1 is not deleted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321 was a gift from George Church (Addgene plasmid # 48999)
  • For your References section:

    Genomically recoded organisms expand biological functions. Lajoie MJ, Rovner AJ, Goodman DB, Aerni HR, Haimovich AD, Kuznetsov G, Mercer JA, Wang HH, Carr PA, Mosberg JA, Rohland N, Schultz PG, Jacobson JM, Rinehart J, Church GM, Isaacs FJ. Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459. 10.1126/science.1241459 PubMed 24136966