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C321.∆A.opt
(Bacterial strain #87359)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 87359 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    C321.∆A.opt strain
  • Growth instructions
    Do not grow above 34 C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C321.∆A.opt strain
  • Species
    Synthetic

Resource Information

Depositor Comments

C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595.

This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction.

The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.∆A.opt was a gift from George Church (Addgene plasmid # 87359)
  • For your References section:

    Optimizing complex phenotypes through model-guided multiplex genome engineering. Kuznetsov G, Goodman DB, Filsinger GT, Landon M, Rohland N, Aach J, Lajoie MJ, Church GM. Genome Biol. 2017 May 25;18(1):100. doi: 10.1186/s13059-017-1217-z. 10.1186/s13059-017-1217-z PubMed 28545477