Skip to main content
Holiday Schedule: Addgene will be closed December 24 - December 31. Order processing and shipping will resume on January 3, 2022. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)
(Plasmid #50863)


Item Catalog # Description Quantity Price (USD)
Plasmid 50863 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA 3.1 neo
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 5316
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human NKCC1 (synthetic)
  • Alt name
    Slc12A2 (synthetic)
  • Alt name
    Na-K-Cl cotransporter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Mutation
    I674C I730C in hNKCC1 in NT17
  • Entrez Gene
    SLC12A2 (a.k.a. BSC, BSC2, NKCC1, PPP1R141)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3x Flag (N terminal on insert)
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid constructed from pcDNA3.1 Flag YFP hNKCC1 WT (NT17). See Addgene plasmid# 49085 ( for plasmid map of wild-type construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835) was a gift from Biff Forbush (Addgene plasmid # 50863 ; ; RRID:Addgene_50863)
  • For your References section:

    Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion Transport and Transporter Activation Revealed by Internal Crosslinking between Transmembrane Domains 10 and 11/12. Monette MY, Somasekharan S, Forbush B. J Biol Chem. 2014 Jan 22. 10.1074/jbc.M113.542258 PubMed 24451383