Skip to main content
Holiday Schedule: Addgene will be closed December 24 - December 31. Order processing and shipping will resume on January 3, 2022. For questions about estimated ship dates, please feel free to track your order status or contact help[email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion Transport and Transporter Activation Revealed by Internal Crosslinking between Transmembrane Domains 10 and 11/12.
Monette MY, Somasekharan S, Forbush B
J Biol Chem. 2014 Jan 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50863pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50864pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50865pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50866pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50867pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50868pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50869pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50870pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50873pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50874pcDNA3.1 Flag YFP hNKCC1 V673C (NT437)Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50875pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50876pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50877pcDNA3.1 Flag YFP hNKCC1 P676C (NT443)Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50878pcDNA3.1 Flag YFP hNKCC1 I677C (NT467)Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50879pcDNA3.1 Flag YFP hNKCC1 I678C (NT445)Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50880pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50881pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50884pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50885pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50889pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50890pcDNA3.1 Flag YFP hNKCC1 L736C (NT522)Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50891pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50905pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50906pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50907pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50909pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50910pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
Loading...
50912pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
Loading...
50913pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
Loading...
50914pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
Loading...