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MSCV-PIG-Cre
(Plasmid #50935)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50935 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV PIG, Addgene plasmid # 18751
  • Backbone manufacturer
    Scott Lowe
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre recombinase
  • Insert Size (bp)
    1000
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer MSCV
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains Puro resistance gene, IRES, and GFP downstream of PGK promoter.
The Nuclear Localization Sequence fusion Cre recombinase (NLS-Cre) was excised from pTZ-CreN vector (a gift from L. Nitschke).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-PIG-Cre was a gift from Pier Pandolfi (Addgene plasmid # 50935 ; http://n2t.net/addgene:50935 ; RRID:Addgene_50935)
  • For your References section:

    Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Chen Z, Trotman LC, Shaffer D, Lin HK, Dotan ZA, Niki M, Koutcher JA, Scher HI, Ludwig T, Gerald W, Cordon-Cardo C, Pandolfi PP. Nature. 2005 Aug 4;436(7051):725-30. 10.1038/nature03918 PubMed 16079851