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ePop
(Plasmid #50953)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50953 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pProTet.E
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    A 40bp oligo with NdeI and NheI sites was introduced into the AatII site of pProTet.E

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please screen at least 5 small colonies by restriction digest immediately upon receipt. Plasmid is likely unstable.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    E lysis protein
  • Species
    phage φX174
  • Insert Size (bp)
    276

Gene/Insert 2

  • Gene/Insert name
    LuxI
  • Species
    V. fischeri
  • Insert Size (bp)
    582
  • Mutation
    mutated luxR
  • GenBank ID
    AY275714.1

Gene/Insert 3

  • Gene/Insert name
    LuxR
  • Species
    Aliivibrio fischeri
  • Insert Size (bp)
    753
  • Mutation
    Early stop codon introduced in mutated luxR results in loss of luxR functionality; the regulation of this gene is not required for oscillating behavior observed in the study
  • GenBank ID

Resource Information

Depositor Comments

Expression strain: MC4100

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ePop was a gift from Lingchong You (Addgene plasmid # 50953 ; http://n2t.net/addgene:50953 ; RRID:Addgene_50953)
  • For your References section:

    Oscillations by minimal bacterial suicide circuits reveal hidden facets of host-circuit physiology. Marguet P, Tanouchi Y, Spitz E, Smith C, You L. PLoS One. 2010 Jul 30;5(7):e11909. doi: 10.1371/journal.pone.0011909. 10.1371/journal.pone.0011909 PubMed 20689598