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AAV-SYP1-miniSOG-T2A-mCherry
(Plasmid #50972)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50972 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV2
  • Backbone size w/o insert (bp) 4575
  • Total vector size (bp) 6027
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Regular growing condition.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synaptophysin-miniSOG-T2A-mCherry
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2070
  • GenBank ID
    BC099798.1
  • Entrez Gene
    Syp (a.k.a. Syp1)
  • Promoter human synapsin promoter
  • Tags / Fusion Proteins
    • T2A-mCherry (C terminal on insert)
    • miniSOG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

WPRE is inserted after the mCherry before SV40 polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-SYP1-miniSOG-T2A-mCherry was a gift from Roger Tsien (Addgene plasmid # 50972 ; http://n2t.net/addgene:50972 ; RRID:Addgene_50972)
  • For your References section:

    Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, Tsien RY. Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022. 10.1016/j.neuron.2013.05.022 PubMed 23889931