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pSLQ1661-sgMUC4-E3(F+E)
(Plasmid #51025)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51025 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSico
  • Total vector size (bp) 8320
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    The depositing lab suggests using Stellar (Clontech) cells for growth and maximal DNA prep yield. The plasmid is provided in NEB Stable cells to reduce the chance of recombination.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    optimized sgRNA
  • Alt name
    sgRNA(F+E)
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    115
  • Entrez Gene
    MUC4 (a.k.a. ASGP, HSA276359, MUC-4)
  • Promoter mouse U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer gacgcgccatctctaggcc
  • 3′ sequencing primer atgcatggcggtaatacggttatc
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

gRNA target sequence GTGGCGTGACCTGTGGATGCTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLQ1661-sgMUC4-E3(F+E) was a gift from Bo Huang & Stanley Qi (Addgene plasmid # 51025)
  • For your References section:

    Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. 10.1016/j.cell.2013.12.001 PubMed 24360272