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pBEVY-GL
(Plasmid #51225)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51225 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YEplac181
  • Backbone manufacturer
    R.D.Gietz (University of Manitoba)
  • Modifications to backbone
    To construct the pBEVY-G series of plasmids, the GAL1,10 promoter was amplified by PCR and inserted into the dephosphorylated SmaI site within the polylinker of YEplac181. The GAL1,10 promoter was inserted into the center of the polylinker in an orientation that regenerated a SmaI site 3′ of the GAL1 side of the promoter. EcoRI and HpaI were used to remove a portion of the parent plasmid sequence and an ADH2 terminator having an EcoRI overhang and a blunt end was inserted to provide a termination function for transcription from the GAL1 side of the bi-directional promoter. The ADH1 terminator was digested with HindIII and was inserted into the dephosphorylated HindIII site of the plasmid, placing a second terminator 3′ of the GAL10 gene. The resulting plasmid was called pBEVY-GL because of its galactose-induction and the LEU2 selection gene.
  • Vector type
    Yeast Expression
  • Promoter GAL1,10
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEVY-GL was a gift from Charles Miller (Addgene plasmid # 51225 ; http://n2t.net/addgene:51225 ; RRID:Addgene_51225)
  • For your References section:

    Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae. Miller CA 3rd, Martinat MA, Hyman LE. Nucleic Acids Res. 1998 Aug 1;26(15):3577-83. 10.1093/nar/26.15.3577 PubMed 9671822