pBEVY-GA
(Plasmid #51227)

• Purpose: (Empty Backbone) bi-directional expression vector for yeast, galactose inducible, ADE2 selection
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.

Backbone

• Vector backbone: pBEVY-GT
• Backbone manufacturer: Addgene plasmid 51231
• Modifications to backbone: pBEVY-GA was made by cutting pBEVY-GT with AatII and XcmI to remove the TRP1 gene. A fragment containing the ADE2 gene derived from a BglII digest of the pASZ11 plasmid was inserted using blunt ligation to generate pBEVY-GA.
• Vector type: Yeast Expression
• Promoter: GAL1,10
• Selectable markers: ADE2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Gal1 and Gal10Pro_F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.