Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #51388)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 51388 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    miR-23a promoter region
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR23A (a.k.a. MIRN23A, hsa-mir-23a, miRNA23A, mir-23a)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3_primer
  • 3′ sequencing primer LucNrev_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

position: -603~+36nt (compared to transcription start site of pri-miR-23a) (sequence: Fig. 4A)

Addgene sequencing has found a T50260C and A50093 insert relative to human chromosome 19 (gb|AC020916.8), this is in the promoter region and is not believed to effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-23P639 was a gift from Narry Kim (Addgene plasmid # 51388 ; ; RRID:Addgene_51388)
  • For your References section:

    MicroRNA genes are transcribed by RNA polymerase II. Lee Y, Kim M, Han J, Yeom KH, Lee S, Baek SH, Kim VN. EMBO J. 2004 Oct 13;23(20):4051-60. Epub 2004 Sep 16. 10.1038/sj.emboj.7600385 PubMed 15372072