Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51388||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemiR-23a promoter region
SpeciesH. sapiens (human)
Entrez GeneMIR23A (a.k.a. MIRN23A, hsa-mir-23a, miRNA23A, mir-23a)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3_primer
- 3′ sequencing primer LucNrev_primer (Common Sequencing Primers)
position: -603~+36nt (compared to transcription start site of pri-miR-23a) (sequence: Fig. 4A)
Addgene sequencing has found a T50260C and A50093 insert relative to human chromosome 19 (gb|AC020916.8), this is in the promoter region and is not believed to effect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-23P639 was a gift from Narry Kim (Addgene plasmid # 51388 ; http://n2t.net/addgene:51388 ; RRID:Addgene_51388)
For your References section:MicroRNA genes are transcribed by RNA polymerase II. Lee Y, Kim M, Han J, Yeom KH, Lee S, Baek SH, Kim VN. EMBO J. 2004 Oct 13;23(20):4051-60. Epub 2004 Sep 16. 10.1038/sj.emboj.7600385 PubMed 15372072