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pGL3-CMV-p85a 3'UTR
(Plasmid #51392)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51392 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-UC
  • Backbone manufacturer
    Promega (modified to have 3' multi cloning sites)
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p85alpha 3'UTR
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA)
  • Promoter CMV
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer EBV_rev_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Inserted sequences: position 165~1679 (cf. full-length 3'UTR: 4220bp); point mutations: substitution (G204C), insertion (983A)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-CMV-p85a 3'UTR was a gift from Narry Kim (Addgene plasmid # 51392 ; http://n2t.net/addgene:51392 ; RRID:Addgene_51392)
  • For your References section:

    miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Park SY, Lee JH, Ha M, Nam JW, Kim VN. Nat Struct Mol Biol. 2009 Jan;16(1):23-9. doi: 10.1038/nsmb.1533. Epub 2008 Dec 14. 10.1038/nsmb.1533 PubMed 19079265