pGL3-CMV-p85a 3'UTR mutant
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51393||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerPromega (modified to have 3' multi cloning sites)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep85alpha 3'UTR
SpeciesH. sapiens (human)
MutationmiR-29 binding site mutant
Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA)
- Promoter CMV
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
miR-29 seed mutant (TGCT->GTAG)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-CMV-p85a 3'UTR mutant was a gift from Narry Kim (Addgene plasmid # 51393 ; http://n2t.net/addgene:51393 ; RRID:Addgene_51393)
For your References section:miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Park SY, Lee JH, Ha M, Nam JW, Kim VN. Nat Struct Mol Biol. 2009 Jan;16(1):23-9. doi: 10.1038/nsmb.1533. Epub 2008 Dec 14. 10.1038/nsmb.1533 PubMed 19079265