|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51492||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 11216
Vector typeplant expression T-DNA
Growth in Bacteria
Copy numberHigh Copy
Speciesbean yellow dwarf virus
Insert Size (bp)1000
Mutationchanged lysine 219 to histidine
- Promoter 2x35S
- Cloning method Gateway Cloning
- 5′ sequencing primer atgccttctgctagtaagaactt
- 3′ sequencing primer aggcacgttcagtgactcga (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byI did not originally clone the gene. Rep/RepA coding sequence was synthesized on g-Blocks from IDT.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pREPK219H was a gift from Daniel Voytas (Addgene plasmid # 51492 ; http://n2t.net/addgene:51492 ; RRID:Addgene_51492)
For your References section:DNA Replicons for Plant Genome Engineering. Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF. Plant Cell. 2014 Jan 17. 10.1105/tpc.113.119792 PubMed 24443519