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HO-Poly-HO
(Plasmid #51661)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51661 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC21-NotI
  • Backbone manufacturer
    Stillman Lab, Addgene plasmid # 51659
  • Modifications to backbone
    A 912 bp fragment (HO-L) with sequences from the HO promoter (–2720 to –1814 upstream from the ATG) was made by PCR with primers designed to generate a fragment with HinDIII and BsiWI overhang sequences. This fragment was inserted into HinDIII–BsiWI digested pUC21-NotI. A 507 bp fragment (HO-R) with sequences from the 3′ end of the HO gene (+1199 to +1699 downstream of the ATG) was made by PCR with primers designed to generate a fragment with EcoRI and SfiI overhang sequences. This fragment was inserted into EcoRI–SfiI digested pUC21-NotI.
  • Vector type
    Yeast Expression ; yeast integration

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HO-Poly-HO was a gift from David Stillman (Addgene plasmid # 51661 ; http://n2t.net/addgene:51661 ; RRID:Addgene_51661)
  • For your References section:

    Yeast vectors for integration at the HO locus. Voth WP, Richards JD, Shaw JM, Stillman DJ. Nucleic Acids Res. 2001 Jun 15;29(12):E59-9. 10.1093/nar/29.12.e59 PubMed 11410682