PurposeStrain for use in Rinehart Lab Phosphoprotein synthesis kit
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||52055||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)None (Empty strain - no antibiotic resistance)
Growth Strain(s)The gene encoding phosphoserine phosphatase (serB) was mutated within the genome of rEc7 delta prfA delta tolC.bla:T7
Growth instructionsStrain grows slowly.
We recommend growth of the EcAR7 strain at 30 °C in LB supplemented with 0.08% glucose. The EcAR7 strains normally take 1.5-‐2 days to grow at 30°C, after transformation or streaking the strain on an agar plate (with the appropriate antibiotics).
See supplemental information from referenced article for protocol on creating EcAR7 from EcNR2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EcAR7 was a gift from Jesse Rinehart (Addgene plasmid # 52055)
For your References section:Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, Rogulina S, Cheng L, Olds W, Fischer JT, Soll D, Isaacs FJ, Rinehart J. FEBS Lett. 2012 Oct 19;586(20):3716-22. doi: 10.1016/j.febslet.2012.08.031. Epub 2012 Sep 13. 10.1016/j.febslet.2012.08.031 PubMed 22982858