|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52137||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5930
Modifications to backboneInsert containing PUM-HD flanking regions 1' and 8', separated by LacZ alpha, cloned using the Gibson Assembly method.
Vector typeBacterial Expression
- Promoter T7
/ Fusion Proteins
- PUM-HD flanking region 1' (N terminal on insert)
- PUM-HD flanking region 8' (C terminal on insert)
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-GG-PUF was a gift from Huimin Zhao (Addgene plasmid # 52137 ; http://n2t.net/addgene:52137 ; RRID:Addgene_52137)
For your References section:Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042