pET28-GG-PUF
(Plasmid #52137)

• Purpose: (Empty Backbone) Receiving vector for PUM-HD assembly and expression in E.coli
• Depositing Lab: Huimin Zhao
• Publication: Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size (bp): 5930
• Modifications to backbone: Insert containing PUM-HD flanking regions 1' and 8', separated by LacZ alpha, cloned using the Gibson Assembly method.
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • PUM-HD flanking region 1' (N terminal on insert)
  • PUM-HD flanking region 8' (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: T7 For
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry