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Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA.
Abil Z, Denard CA, Zhao H
J Biol Eng. 2014 Mar 1;8(1):7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52136pChlr-pNEB193A backbone plasmid for creation of additional PUF repeat modules
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52137pET28-GG-PUFReceiving vector for PUM-HD assembly and expression in E.coli
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52138pCMV-TTP(C147R)-GG-PUFReceiving vector for PUM-HD assembly and expression in mammalian cells
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52139pChlr-1SQEncodes module 1 with amino acids 12S-16Q for recognition of nt 8A
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52140pChlr-1SEEncodes module 1 with amino acids 12S-16E for recognition of nt 8G
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52141pChlr-1NQEncodes module 1 with amino acids 12N-16Q for recognition of nt 8U
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52142pChlr-1SREncodes module 1 with amino acids 12S-16R for recognition of nt 8C
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52143pChlr-2CQEncodes module 2 with amino acids 12C-16Q for recognition of nt 7A
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52144pChlr-2SEEncodes module 2 with amino acids 12S-16E for recognition of nt 7G
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52145pChlr-2NQEncodes module 2 with amino acids 12N-16Q for recognition of nt 7U
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52146pChlr-2SREncodes module 2 with amino acids 12S-16R for recognition of nt 7C
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52147pChlr-3CQEncodes module 3 with amino acids 12C-16Q for recognition of nt 6A
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52148pChlr-3SEEncodes module 3 with amino acids 12S-16E for recognition of nt 6G
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52149pChlr-3NQEncodes module 3 with amino acids 12N-16Q for recognition of nt 6U
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52150pChlr-3SREncodes module 3 with amino acids 12S-16R for recognition of nt 6C
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52151pChlr-4CQEncodes module 4 with amino acids 12C-16Q for recognition of nt 5A
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52152pChlr-4SEEncodes module 4 with amino acids 12S-16E for recognition of nt 5G
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52153pChlr-4NQEncodes module 4 with amino acids 12N-16Q for recognition of nt 5U
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52154pChlr-4SREncodes module 4 with amino acids 12S-16R for recognition of nt 5C
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52155pChlr-4CYQEncodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A
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52156pChlr-4SYEEncodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G
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52157pChlr-4NYQEncodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U
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52158pChlr-4SYREncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
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52159pChlr-5CQEncodes module 5 with amino acids 12C-16Q for recognition of nt 4A
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52160pChlr-5SEEncodes module 5 with amino acids 12S-16E for recognition of nt 4G
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52161pChlr-5NQEncodes module 5 with amino acids 12N-16Q for recognition of nt 4U
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52162pChlr-5SREncodes module 5 with amino acids 12S-16R for recognition of nt 4C
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52163pChlr-6CQEncodes module 6 with amino acids 12C-16Q for recognition of nt 3A
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52164pChlr-6SEEncodes module 6 with amino acids 12S-16E for recognition of nt 3G
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52165pChlr-6NQEncodes module 6 with amino acids 12N-16Q for recognition of nt 3U
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52166pChlr-6SREncodes module 6 with amino acids 12S-16R for recognition of nt 3C
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52167pChlr-7CQEncodes module 7 with amino acids 12C-16Q for recognition of nt 2A
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52168pChlr-7SEEncodes module 7 with amino acids 12S-16E for recognition of nt 2G
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52169pChlr-7NQEncodes module 7 with amino acids 12N-16Q for recognition of nt 2U
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52170pChlr-7SYREncodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C
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52171pChlr-8CQEncodes module 8 with amino acids 12C-16Q for recognition of nt 1A
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52172pChlr-8SEEncodes module 8 with amino acids 12S-16E for recognition of nt 1G
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52173pChlr-8NQEncodes module 8 with amino acids 12N-16Q for recognition of nt 1U
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52174pChlr-8SREncodes module 8 with amino acids 12S-16R for recognition of nt 1C
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