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pChlr-6NQ
(Plasmid #52165)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52165 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pChlr-pNEB193
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 2280
  • Total vector size (bp) 2340
  • Modifications to backbone
    Replaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pumilio homology domain amino acids 205-240
  • Alt name
    PUM-HD module 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    36
  • GenBank ID
    D43951
  • Entrez Gene
    PUM1 (a.k.a. HSPUM, PUMH, PUMH1, PUML1, SCA47)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pChlr-6NQ was a gift from Huimin Zhao (Addgene plasmid # 52165 ; http://n2t.net/addgene:52165 ; RRID:Addgene_52165)
  • For your References section:

    Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042