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pChlr-pNEB193
(Plasmid #52136)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52136 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNEB193
  • Backbone manufacturer
    New England Biolabs
  • Backbone size (bp) 2280
  • Modifications to backbone
    Replaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the plasmid contains a 1.3 kb insert containing Transposon 10 between bp# 51-52 of the original plasmid sequence. This insertion was not intentional, but does not affect the use of the plasmid to generate additional PUF repeat modules. Alternatively, any other PUF repeat plasmid can be similarly used to create new PUF repeate modules, since they all have the necessary SacI and HindIII sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pChlr-pNEB193 was a gift from Huimin Zhao (Addgene plasmid # 52136 ; http://n2t.net/addgene:52136 ; RRID:Addgene_52136)
  • For your References section:

    Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042