|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52136||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England Biolabs
- Backbone size (bp) 2280
Modifications to backboneReplaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the plasmid contains a 1.3 kb insert containing Transposon 10 between bp# 51-52 of the original plasmid sequence. This insertion was not intentional, but does not affect the use of the plasmid to generate additional PUF repeat modules. Alternatively, any other PUF repeat plasmid can be similarly used to create new PUF repeate modules, since they all have the necessary SacI and HindIII sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pChlr-pNEB193 was a gift from Huimin Zhao (Addgene plasmid # 52136 ; http://n2t.net/addgene:52136 ; RRID:Addgene_52136)
For your References section:Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042